APOBEC1 Complementation Factor (A1CF) (C-Term), (AA 394-423) antibody

Details for Product No. ABIN654627
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Antigen
Synonyms A1CF, ACF, ACF64, ACF65, APOBEC1CF, ASP, 1810073H04Rik, Acf, A1cft, Apobec-1, acf, acf64, acf65, apobec1cf, asp
Epitope
C-Term, AA 394-423
(8), (8), (8), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(36), (15), (15), (12), (11), (1), (1), (1)
Host
Rabbit
(34), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(27), (11), (9), (9), (8), (6), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN654627
291.50 $
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Immunogen This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 AA from the C-terminal region of human ACF.
Clone RB20614
Isotype Ig
Specificity This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-427 amino acids from the C-terminal region of human ACF.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ACF
Background Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.
Synonyms: APOBEC1 complementation factor,A1CF,ACF, ASP
Molecular Weight 65202 DA
Gene ID 29974
NCBI Accession NP_620310
UniProt Q9NQ94
Research Area Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-APOBEC1 Complementation Factor (A1CF) (C-Term), (AA 394-423) antibody ACF Antibody (C-term) (ABIN654627) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Blanc, Kennedy, Davidson: "A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 41198-204, 2003 (PubMed).

Xie, Sowden, Dance et al.: "The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 21, pp. 8114-9, 2004 (PubMed).

Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10. ..." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).

Blanc, Henderson, Newberry et al.: "Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality." in: Molecular and cellular biology, Vol. 25, Issue 16, pp. 7260-9, 2005 (PubMed).

Galloway, Smith: "The expression of apoB mRNA editing factors is not the sole determinant for the induction of editing in differentiating Caco-2 cells." in: Biochemical and biophysical research communications, Vol. 391, Issue 1, pp. 659-63, 2010 (PubMed).

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