|+1 404 474 4654|
|+1 888 205 9894 (TF)|
APOBEC1 Complementation Factor (A1CF) (C-Term) antibody
|Synonyms||ACF, ASP, ACF64, ACF65, APOBEC1CF, MGC163391, RP11-564C4.2, Acf, 1810073H04Rik, A1cft, Apobec-1, A1CF, DKFZp469H226, acf, asp, acf64, acf65, MGC81834, apobec1cf|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-427 amino acids from the C-terminal region of human ACF.|
|Description||Other names: APOBEC1 complementation factor,A1CF,ACF, ASP|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-427 amino acids from the C-terminal region of human ACF.|
|Molecular Weight||65202 DA|
Background: Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Transcription Factors, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
|ACF Antibody (C-term) (ABIN654627) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.|
Blanc, Kennedy, Davidson: "A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 41198-204, 2003 (PubMed).
Xie, Sowden, Dance et al.: "The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 21, pp. 8114-9, 2004 (PubMed).
Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).
Blanc, Henderson, Newberry et al.: "Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality." in: Molecular and cellular biology, Vol. 25, Issue 16, pp. 7260-9, 2005 (PubMed).
Galloway, Smith: "The expression of apoB mRNA editing factors is not the sole determinant for the induction of editing in differentiating Caco-2 cells." in: Biochemical and biophysical research communications, Vol. 391, Issue 1, pp. 659-63, 2010 (PubMed).
|Reactivities||Human (25), Mouse (Murine) (19), Rat (Rattus) (19), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Xenopus laevis (1)|
|Applications||Immunofluorescence (IF) (14), Western Blotting (WB) (12), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (2), N-Term (2)|