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Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 399-427), (Center) antibody

Details for Product No. ABIN654630, Supplier: Log in to see
Antigen
  • AI608257
  • bHLHd2
  • lop13
  • nuc
  • SREBF2
  • Srebf2_retired
  • SREBP-2
  • srebp2
  • SREBP2
  • SREBP2gc
  • zgc:158371
Epitope
AA 399-427, Center
17
16
12
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
59
18
17
3
3
3
2
1
1
Host
Rabbit
53
7
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
54
28
27
13
10
6
5
2
2
1
Supplier
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Immunogen This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 AA from the Central region of human SREBF2.
Clone RB24513
Isotype Ig
Specificity This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-431 amino acids from the Central region of human SREBF2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SREBF2 (SREBF2 Antibody Abstract)
Background SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. LS-B1609 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, Hep-G2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.
Synonyms: Sterol regulatory element-binding protein 2,SREBF2,BHLHD2, SREBP2
Molecular Weight 123688 DA
Gene ID 6721
NCBI Accession NP_004590
UniProt Q12772
Research Area Translation Factors, Phospho-specific antibodies, Cell Structure, Protein Modifications, Transcription Factors, Signaling, Metabolism
Application Notes IF = 1:10-50, WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Immunofluorescence (IF) image for anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 399-427), (Center) antibody (ABIN654630) Confocal immunofluorescent analysis of SREBF2 Antibody (Center) (ABIN654630) with Hep...
Background publications Gerin, Clerbaux, Haumont, Lanthier, Das, Burant, Leclercq, MacDougald, Bommer: "Expression of miR-33 from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 33652-61, 2010

Marquart, Allen, Ory, Baldán: "miR-33 links SREBP-2 induction to repression of sterol transporters." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 27, pp. 12228-32, 2010

Najafi-Shoushtari, Kristo, Li, Shioda, Cohen, Gerszten, Näär: "MicroRNA-33 and the SREBP host genes cooperate to control cholesterol homeostasis." in: Science (New York, N.Y.), Vol. 328, Issue 5985, pp. 1566-9, 2010