HLA-B antibody (AA 182-208)

Catalog No. ABIN654636

Product Details anti-HLA-B Antibody

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Binding Specificity: AA 182-208

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-B antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 amino acids from the Central region of human HLA-B.

Clone: RB28442

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Alternative Name: HLA-B (HLA-B Products)

Synonyms: AS antibody, HLAB antibody, SPDA1 antibody, mhc1b antibody, major histocompatibility complex, class I, B antibody, major histocompatibility complex nonclassical class Ib L homeolog antibody, HLA-B antibody, BOLA-B antibody, MAMU-B18 antibody, DKFZP469N1225 antibody, mhc1b.L antibody

Background: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

Molecular Weight: 40460

Gene ID: 3106

NCBI Accession: NP_005505

UniProt: P01889

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints