UDP-Gal:betaGlcNAc beta 1,4 Galactosyltransferase, Polypeptide 6 (B4GALT6) (C-Term) antibody

Antigen: UDP-Gal:betaGlcNAc beta 1,4 Galactosyltransferase, Polypeptide 6 (B4GALT6)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654654

Additional Information

Alternative name: B4GALT6

Gene ID: 9331

UniProt: Q9UBX8, NP_004766.2

Immunogen: This B4GALT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the C-terminal region of human B4GALT6.

Isotype: Ig

Description: Other names: Beta-1,4-galactosyltransferase 6,B4GALT6,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This B4GALT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the C-terminal region of human B4GALT6.

Molecular Weight: 44914 DA

Comments:

Background: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose, all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

B4GALT6 Antibody (C-term) (ABIN654654) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Takizawa, Nomura, Wakisaka et al.: "cDNA cloning and expression of human lactosylceramide synthase." in: Biochimica et biophysica acta, Vol. 1438, Issue 2, pp. 301-4, 1999 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Landers, Melki, Meininger et al.: "Reduced expression of the Kinesin-Associated Protein 3 (KIFAP3) gene increases survival in sporadic amyotrophic lateral sclerosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 22, pp. 9004-9, 2009 (PubMed).