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HIST1H2B (AA 57-86), (Center) antibody

Details for Product No. ABIN654684
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Antigen
Epitope
AA 57-86, Center
Reactivity
Human
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN654684
291.50 $
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Immunogen This HIST1H2BJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 AA from the Central region of human HIST1H2BJ.
Clone RB28555
Isotype Ig
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine),Monkey,Rat (Rattus),Xenopus laevis,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].
Synonyms: Histone H2B type 1-J,HIST1H2BJ,H2BFR
Molecular Weight 13904 DA
Gene ID 8970
UniProt NP_066402.2, P06899
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes IHC = 1:50-100, FACS = 1:10-50, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-HIST1H2B (AA 57-86), (Center) antibody HIST1H2BJ antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2BJ antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-HIST1H2B (AA 57-86), (Center) antibody (2) HIST1H2BJ Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-HIST1H2B (AA 57-86), (Center) antibody (3) HIST1H2BJ Antibody (Center) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2BJ antibody detected the HIST1H2BJ protein (arrow).
Background publications Benyamin, McRae, Zhu et al.: "Variants in TF and HFE explain approximately 40% of genetic variation in serum-transferrin levels." in: American journal of human genetics, Vol. 84, Issue 1, pp. 60-5, 2009 (PubMed).

Shi, Levinson, Duan et al.: "Common variants on chromosome 6p22.1 are associated with schizophrenia." in: Nature, Vol. 460, Issue 7256, pp. 753-7, 2009 (PubMed).

Validation Images
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