Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody

Details for Product No. ABIN654898
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Antigen
Synonyms ICE(rel)III, ICEREL-III, ICH-3, CASP5
Epitope
AA 162-191, Center
(12), (11), (8), (7), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(97), (16), (16), (15), (11), (11), (4), (4), (3)
Host
Rabbit
(94), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(89), (42), (12), (11), (10), (9), (9), (6), (3), (3), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN654898
291.50 $
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Immunogen This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 AA from the Central region of human CASP5.
Clone RB18988
Isotype Ig
Specificity This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human CASP5.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CASP5
Background This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein, this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.
Synonyms: Caspase-5,CASP5,ICH3
Molecular Weight 49736 DA
Gene ID 838
NCBI Accession NP_001129584, NP_004338, NP_001129581, NP_001129582
UniProt P51878
Research Area Apoptosis/Necrosis, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody CASP5 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CASP5 antibody detected the CASP5 protein (arrow).
anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody (2) CASP5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody (3) CASP5 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Eckhart, Kittel, Gawlas et al.: "Identification of a novel exon encoding the amino-terminus of the predominant caspase-5 variants." in: Biochemical and biophysical research communications, Vol. 348, Issue 2, pp. 682-8, 2006 (PubMed).

Ulybina, Kuligina, Mitiushkina et al.: "Evidence for depletion of CASP5 Ala90Thr heterozygous genotype in aged subjects." in: Experimental gerontology, Vol. 45, Issue 9, pp. 726-9, 2010 (PubMed).

Validation Images
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