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Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody

Details for Product No. ABIN654898, Supplier: Login to see
Antigen
  • ICE(rel)III
  • ICEREL-III
  • ICH-3
  • CASP5
Epitope
AA 162-191, Center
21
16
15
13
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
97
6
6
4
4
4
2
Host
Rabbit
94
3
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
3
3
3
3
3
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
46
18
16
8
7
4
3
3
2
2
1
Supplier
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Immunogen This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 AA from the Central region of human CASP5.
Clone RB18988
Isotype Ig
Specificity This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human CASP5.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CASP5 (CASP5 Antibody Abstract)
Background This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein, this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.
Synonyms: Caspase-5,CASP5,ICH3
Molecular Weight 49736 DA
Gene ID 838
NCBI Accession NP_001129584, NP_004338, NP_001129581, NP_001129582
UniProt P51878
Research Area Apoptosis/Necrosis, Metabolism, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody (ABIN654898) CASP5 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/l...
Immunohistochemistry (IHC) image for anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody (ABIN654898) CASP5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin...
Flow Cytometry (FACS) image for anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (AA 162-191), (Center) antibody (ABIN654898) CASP5 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram...
Background publications Ulybina, Kuligina, Mitiushkina et al.: "Evidence for depletion of CASP5 Ala90Thr heterozygous genotype in aged subjects." in: Experimental gerontology, Vol. 45, Issue 9, pp. 726-9, 2010 (PubMed).

Eckhart, Kittel, Gawlas et al.: "Identification of a novel exon encoding the amino-terminus of the predominant caspase-5 variants." in: Biochemical and biophysical research communications, Vol. 348, Issue 2, pp. 682-8, 2006 (PubMed).