Nucleolar and Spindle Associated Protein 1 (NUSAP1) (N-Term), (AA 27-56) antibody

Details for Product No. ABIN654953
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Antigen
Epitope
N-Term, AA 27-56
(8), (8), (7), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(45), (15), (13), (12), (11), (1), (1), (1), (1)
Host
Rabbit
(44), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(35), (22), (9), (9), (5), (4), (2), (1)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN654953
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Immunogen This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 AA from the N-terminal region of human NUSAP.
Clone RB25410
Isotype Ig
Specificity This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human NUSAP.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NUSAP
Background NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).
Synonyms: Nucleolar and spindle-associated protein 1,NUSAP1,ANKT
Molecular Weight 49452 DA
Gene ID 51203
NCBI Accession NP_060924, NP_057443, NP_001123369
UniProt Q9BXS6
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Nucleolar and Spindle Associated Protein 1 (NUSAP1) (N-Term), (AA 27-56) antibody NUSAP Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NUSAP antibody detected the NUSAP protein (arrow).
anti-Nucleolar and Spindle Associated Protein 1 (NUSAP1) (N-Term), (AA 27-56) antibody (2) NUSAP antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUSAP antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Background publications Raemaekers, Ribbeck, Beaudouin et al.: "NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization." in: The Journal of cell biology, Vol. 162, Issue 6, pp. 1017-29, 2003 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Wadia, Coram, Armstrong et al.: "Antibodies specifically target AML antigen NuSAP1 after allogeneic bone marrow transplantation." in: Blood, Vol. 115, Issue 10, pp. 2077-87, 2010 (PubMed).

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