Chromosome 20 Open Reading Frame 7 (C20orf7) (C-Term) antibody

Antigen: Chromosome 20 Open Reading Frame 7 (C20orf7)

Synonyms: FLJ22324, MGC90272, dJ842G6.1, bA526K24.2, C20orf7, DKFZp468A017

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654967

Additional Information

Alternative name: C20orf7

Gene ID: 79133

UniProt: Q5TEU4, NP_077025.2, NP_001034464.1

Immunogen: This C20orf7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-323 amino acids from the C-terminal region of human C20orf7.

Isotype: Ig

Description: Other names: Probable methyltransferase C20orf7, mitochondrial,C20orf7,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This C20orf7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-323 amino acids from the C-terminal region of human C20orf7.

Molecular Weight: 38918 DA

Comments:

Background: The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

C20orf7 Antibody (C-term) (ABIN654967) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the C20orf7 antibody detected the C20orf7 protein (arrow).

Publications

Product: Wang, Yoon, Oh et al.: "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1022-32, 2006 (PubMed).

Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).

Sugiana, Pagliarini, McKenzie et al.: "Mutation of C20orf7 disrupts complex I assembly and causes lethal neonatal mitochondrial disease." in: American journal of human genetics, Vol. 83, Issue 4, pp. 468-78, 2008 (PubMed).

Gerards, Sluiter, van den Bosch et al.: "Defective complex I assembly due to C20orf7 mutations as a new cause of Leigh syndrome." in: Journal of medical genetics, Vol. 47, Issue 8, pp. 507-12, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).