Inositol Polyphosphate-5-Phosphatase, 72 KDa (INPP5E) (Center) antibody

Antigen: Inositol Polyphosphate-5-Phosphatase, 72 KDa (INPP5E)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655177

Additional Information

Alternative name: INPP5E

Gene ID: 56623

UniProt: Q9NRR6, NP_063945

Immunogen: This INPP5E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-284 amino acids from the Central region of human INPP5E.

Isotype: Ig

Description: Other names: INPP5E,72 kDa inositol polyphosphate 5-phosphatase,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This INPP5E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-284 amino acids from the Central region of human INPP5E.

Molecular Weight: 70205 DA

Comments:

Background: The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4, 5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology, Protein Modifications, Metabolism, Phospho-specific antibodies

Restrictions: For Research Use only

Publications

Product: Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Jacoby, Cox, Gayral et al.: "INPP5E mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in human and mouse." in: Nature genetics, Vol. 41, Issue 9, pp. 1027-31, 2009 (PubMed).

Bielas, Silhavy, Brancati et al.: "Mutations in INPP5E, encoding inositol polyphosphate-5-phosphatase E, link phosphatidyl inositol signaling to the ciliopathies." in: Nature genetics, Vol. 41, Issue 9, pp. 1032-6, 2009 (PubMed).