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Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 219-248)

Details for Product anti-CASP3 Antibody No. ABIN655271, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
Alternatives
anti-Human Caspase 3 antibody for Western Blotting
Epitope
AA 219-248, C-Term
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30
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30
19
19
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14
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1
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Reactivity
Human
453
190
140
28
18
14
14
14
12
8
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4
1
1
Host
Rabbit
442
67
16
2
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Clonality (Clone)
Polyclonal ()
Conjugate
This Caspase 3 antibody is un-conjugated
24
21
17
10
9
9
4
3
2
2
2
2
2
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2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
368
193
161
87
63
58
35
25
23
21
13
10
7
3
2
2
2
2
2
2
1
1
1
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1
Supplier
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Immunogen This CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 AA from the C-terminal region of human CASP3.
Clone RB14883
Isotype Ig
Specificity This CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human CASP3.
Predicted Reactivity Mouse (Murine),Monkey,Rat (Rattus),Rabbit
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CASP3 (CASP3 Antibody Abstract)
Background This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].
Synonyms: Caspase-3,CASP3,CPP32
Molecular Weight 31608 DA
Gene ID 836
NCBI Accession NP_004337, NP_116786
UniProt P42574
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 219-248) (ABIN655271) CASP3 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/l...
Immunohistochemistry (IHC) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 219-248) (ABIN655271) CASP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin ...
Flow Cytometry (FACS) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 219-248) (ABIN655271) CASP3 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram...
Background publications Srikanth, Wall, Maldonado-Contreras et al.: "Salmonella pathogenesis and processing of secreted effectors by caspase-3." in: Science (New York, N.Y.), Vol. 330, Issue 6002, pp. 390-3, 2010 (PubMed).

Würstle, Laussmann, Rehm: "The caspase-8 dimerization/dissociation balance is a highly potent regulator of caspase-8, -3, -6 signaling." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33209-18, 2010 (PubMed).

Burke, Smith, Smith: "cIAP1 cooperatively inhibits procaspase-3 activation by the caspase-9 apoptosome." in: The Journal of biological chemistry, Vol. 285, Issue 39, pp. 30061-8, 2010 (PubMed).