Thiamine Triphosphatase (THTPA) (C-Term) antibody

Antigen: Thiamine Triphosphatase (THTPA)

Synonyms: THTP, MGC2652, THTPASE, AW122966, MGC38198, MGC128756, MGC94155, MGC130853, DKFZp459H018

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655595

Additional Information

Alternative name: THTPA

Gene ID: 79178

UniProt: Q9BU02, NP_077304.1, NP_001119811.1

Immunogen: This THTPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-230 amino acids from the C-terminal region of human THTPA.

Isotype: Ig

Description: Other names: Thiamine-triphosphatase,THTPA,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This THTPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-230 amino acids from the C-terminal region of human THTPA.

Molecular Weight: 25566 DA

Comments:

Background: Thiamine triphosphatase (THTPA) catalyzes the hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), which is the major cellular form of thiamine (vitamin B1) (Lakaye et al., 2002 [PubMed 11827967]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Protein Modifications, Signaling, Metabolism

Restrictions: For Research Use only

Images

THTPA Antibody (C-term) (ABIN655595) western blot analysis in MDA-MB231,NCI-H460,HL-60 cell line lysates (35ug/lane). This demonstrates the THTPA antibody detected the THTPA protein (arrow).

Publications

Product: Lakaye, Makarchikov, Antunes et al.: "Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13771-7, 2002 (PubMed).

Heilig, Eckenberg, Petit et al.: "The DNA sequence and analysis of human chromosome 14." in: Nature, Vol. 421, Issue 6923, pp. 601-7, 2003 (PubMed).