Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3) (Center) antibody

Antigen: Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3)

Synonyms: DD3, DDX, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, AKR1C3, PGFS, MGC134333, DKFZp469P081

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655603

Additional Information

Alternative name: AKR1C3

Gene ID: 8644

UniProt: NP_003730.4, P42330

Immunogen: This AKR1C3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the Central region of human AKR1C3.

Isotype: Ig

Description: Other names: Aldo-keto reductase family 1 member C3,AKR1C3,DDH1, HSD17B5, KIAA0119, PGFS

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AKR1C3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the Central region of human AKR1C3.

Molecular Weight: 36853 DA

Comments:

Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

AKR1C3 Antibody (Center) (ABIN655603) western blot analysis in Hela,HepG2 cell line lysates (35ug/lane). This demonstrates the AKR1C3 antibody detected the AKR1C3 protein (arrow).

Publications

Product: Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Canzian, Cox, Setiawan et al.: "Comprehensive analysis of common genetic variation in 61 genes related to steroid hormone and insulin-like growth factor-I metabolism and breast cancer risk in the NCI breast and prostate cancer cohort consortium." in: Human molecular genetics, Vol. 19, Issue 19, pp. 3873-84, 2010 (PubMed).

Zakharov, Lin, Azzarello et al.: "Suppressed expression of type 2 3alpha/type 5 17beta-hydroxysteroid dehydrogenase (AKR1C3) in endometrial hyperplasia and carcinoma." in: International journal of clinical and experimental pathology, Vol. 3, Issue 6, pp. 608-17, 2010 (PubMed).

Wang, Chorley, Pittman et al.: "Genetic variation and antioxidant response gene expression in the bronchial airway epithelium of smokers at risk for lung cancer." in: PLoS ONE, Vol. 5, Issue 8, pp. e11934, 2010 (PubMed).