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SSH3 antibody (Slingshot Homolog 3 (Drosophila)) (AA 575-602)

Details for Product anti-SSH3 Antibody No. ABIN655643, Supplier: Log in to see
Antigen
  • SSH3
  • BC028922
  • SSH-3
  • mSSH-3L
  • SSH3L
Epitope
AA 575-602, C-Term
17
16
5
3
3
3
2
2
2
2
1
1
Reactivity
Human
44
8
8
Host
Rabbit
36
8
Clonality (Clone)
Polyclonal ()
Conjugate
This SSH3 antibody is un-conjugated
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
38
29
26
18
16
7
6
6
1
1
Supplier
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Immunogen This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 AA from the C-terminal region of human SSH3.
Clone RB19876
Isotype Ig
Specificity This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of human SSH3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SSH3 (SSH3 Antibody Abstract)
Background The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1, MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
Synonyms: RB19876
Pathways: Regulation of actin cytoskeleton
Molecular Weight 72996 Da
Gene ID 54961
UniProt Q8TE77
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.28 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-SSH3 antibody (Slingshot Homolog 3 (Drosophila)) (AA 575-602) (ABIN655643) SSH3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).T...
Immunohistochemistry (IHC) image for anti-SSH3 antibody (Slingshot Homolog 3 (Drosophila)) (AA 575-602) (ABIN655643) SSH3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin e...
Flow Cytometry (FACS) image for anti-SSH3 antibody (Slingshot Homolog 3 (Drosophila)) (AA 575-602) (ABIN655643) SSH3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) comp...
Immunofluorescence (IF) image for anti-SSH3 antibody (Slingshot Homolog 3 (Drosophila)) (AA 575-602) (ABIN655643) Confocal immunofluorescent analysis of SSH3 Antibody (C-term) with Hela cell followed...
Background publications Kligys, Claiborne, DeBiase et al.: "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes." in: The Journal of biological chemistry, Vol. 282, Issue 44, pp. 32520-8, 2007 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).