Immediate Early Response 3 (IER3) (N-Term), (AA 38-65) antibody

Details for Product No. ABIN655735
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Antigen
Epitope
N-Term, AA 38-65
(8), (7), (4), (3), (2), (1)
Reactivity
Human, Mouse (Murine)
(39), (27), (16), (16), (16), (16)
Host
Rabbit
(39)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(24), (16), (13), (13), (10), (9), (7), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN655735
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Immunogen This IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 AA from the N-terminal region of human IER3.
Clone RB20475
Isotype Ig
Specificity This IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-73 amino acids from the N-terminal region of human IER3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name IER3
Background This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein.
Synonyms: IER3,Radiation-inducible immediate-early gene IEX-1,DIF2, IEX1, PRG1
Molecular Weight 16929 DA
Gene ID 8870
NCBI Accession NP_003888
UniProt P46695
Research Area Cell Signaling, Cell Structure, Protein Modifications
Application Notes WB = 1:1000, IHC = 1:50-100, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.36 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Immediate Early Response 3 (IER3) (N-Term), (AA 38-65) antibody IER3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).
anti-Immediate Early Response 3 (IER3) (N-Term), (AA 38-65) antibody (2) IER3 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).
anti-Immediate Early Response 3 (IER3) (N-Term), (AA 38-65) antibody (3) IER3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IER3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Immediate Early Response 3 (IER3) (N-Term), (AA 38-65) antibody (4) Confocal immunofluorescent analysis of IER3 Antibody (N-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
anti-Immediate Early Response 3 (IER3) (N-Term), (AA 38-65) antibody (5) IER3 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Do, Ucisik-Akkaya, Davis et al.: "TP53 R72P and MDM2 SNP309 polymorphisms in modification of childhood acute lymphoblastic leukemia susceptibility." in: Cancer genetics and cytogenetics, Vol. 195, Issue 1, pp. 31-6, 2009 (PubMed).

Fellay, Ge, Shianna et al.: "Common genetic variation and the control of HIV-1 in humans." in: PLoS genetics, Vol. 5, Issue 12, pp. e1000791, 2009 (PubMed).

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