Smoothelin (SMTN) (C-Term) antibody

Antigen: Smoothelin (SMTN)

Synonyms: FLJ35365, FLJ38597, smsmo, MGC68765, SMTN, MGC140000, MGC155237, DKFZp468D019

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655907

Additional Information

Alternative name: SMTN

Gene ID: 6525

UniProt: P53814, NP_008863

Immunogen: This SMTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 884-917 amino acids from the C-terminal region of human SMTN.

Isotype: Ig

Description: Other names: SMTN,Smoothelin,SMSMO

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SMTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 884-917 amino acids from the C-terminal region of human SMTN.

Molecular Weight: 99462 DA

Comments:

Background: This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in three transcript variants. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

SMTN Antibody (C-term) (ABIN655907) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMTN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Council, Hameed: "Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 5, pp. 639-50, 2009 (PubMed).

Coco, Hirsch, Hornick: "Smoothelin is a specific marker for smooth muscle neoplasms of the gastrointestinal tract." in: The American journal of surgical pathology, Vol. 33, Issue 12, pp. 1795-801, 2009 (PubMed).