RECQL2 (ARABIDOPSIS RECQ HELICASE L2); 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) (Center, Thr802) antibody

Antigen: RECQL2 (ARABIDOPSIS RECQ HELICASE L2); 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2)

Synonyms: RECQ3, RECQL2, RECQL3, DKFZp686C2056, ARABIDOPSIS RECQ HELICASE L2, ARABIDOPSIS THALIANA RECQ 2, ATRECQ2, T19E23.16, T19E23_16, xBLM, recq2, recql2, recql3

Binding Site: Center, Thr802

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656017

Additional Information

Alternative name: WRN

Gene ID: 7486

UniProt: NP_000544.2, Q14191

Immunogen: This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-825 amino acids from the Central region of human WRN.

Isotype: Ig

Description: Other names: WRN,Werner syndrome ATP-dependent helicase,RECQ3, RECQL2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-825 amino acids from the Central region of human WRN.

Molecular Weight: 162495 DA

Comments:

Background: This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Perry, Asaithamby, Barnebey et al.: "Identification of a coiled coil in werner syndrome protein that facilitates multimerization and promotes exonuclease processivity." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25699-707, 2010 (PubMed).