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Mediator Complex Subunit 6 (MED6) (AA 217-246), (C-Term) antibody

Details for Product No. ABIN656028, Supplier: Log in to see
Antigen
  • NY-REN-28
  • wu:fa14c11
  • zgc:86933
  • 1500012F11Rik
  • AU019660
  • AV213303
Epitope
AA 217-246, C-Term
18
16
16
15
10
8
5
3
2
2
2
1
Reactivity
Human
70
10
9
2
2
2
Host
Rabbit
57
13
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
65
37
2
2
2
1
Supplier
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Immunogen This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 AA from the C-terminal region of human MED6.
Clone RB20184
Isotype Ig
Specificity This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-246 amino acids from the C-terminal region of human MED6.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name MED6 (MED6 Antibody Abstract)
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Synonyms: MED6,Mediator of RNA polymerase II transcription subunit 6,ARC33
Molecular Weight 28425 DA
Gene ID 10001
NCBI Accession NP_005457
UniProt O75586
Research Area Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.29 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Mediator Complex Subunit 6 (MED6) (AA 217-246), (C-Term) antibody (ABIN656028) MED6 Antibody (C-term) (ABIN656028) western blot analysis in HepG2 cell line lysates ...
Background publications Pavri, Lewis, Kim et al.: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).

Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Yang, DeBeaumont, Zhou et al.: "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2339-44, 2004 (PubMed).

Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Sato, Tomomori-Sato, Banks et al.: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).