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Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody

Details for Product No. ABIN656092
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Antigen
Epitope
C-Term, AA 366-393
(8), (7), (5), (2), (2), (1)
Reactivity
Human
(22), (6), (4)
Host
Rabbit
(18), (4), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(21), (14), (8), (7), (6), (4), (3), (2), (2)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN656092
291.50 $
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Immunogen This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 AA from the C-terminal region of human NKD2.
Clone RB31974
Isotype Ig
Specificity This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-401 amino acids from the C-terminal region of human NKD2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NKD2
Background In the mouse, Nkd is a Dishevelled (see DVL1, MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1, MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.
Synonyms: NKD2,Protein naked cuticle homolog 2,
Molecular Weight 50055 DA
Gene ID 85409
UniProt NP_149111.1, Q969F2
Research Area Ubiquitin-related antibodies, Cell Signaling, Cell Structure, Protein Modifications
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody NKD2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NKD2 antibody detected the NKD2 protein (arrow).
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody (2) NKD2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NKD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody (3) Confocal immunofluorescent analysis of NKD2 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody (4) NKD2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Background publications Li, Franklin, Graves-Deal et al.: "Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 15, pp. 5571-6, 2004 (PubMed).

Adamowicz, Radlwimmer, Rieker et al.: "Frequent amplifications and abundant expression of TRIO, NKD2, and IRX2 in soft tissue sarcomas." in: Genes, chromosomes & cancer, Vol. 45, Issue 9, pp. 829-38, 2006 (PubMed).

Li, Hao, Cao et al.: "Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells." in: Molecular biology of the cell, Vol. 18, Issue 8, pp. 3081-93, 2007 (PubMed).

Ding, Li, Hu et al.: "EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 36, pp. 13433-8, 2008 (PubMed).

Hu, Li, Cao et al.: "Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane." in: The Journal of biological chemistry, Vol. 285, Issue 18, pp. 13561-8, 2010 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN656092
291.50 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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