Did you know that you can buy products from over 140 different suppliers from us?

Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody

Details for Product No. ABIN656092
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
C-Term, AA 366-393
(8), (7), (5), (2), (2), (1)
Reactivity
Human
(22), (6), (4)
Host
Rabbit
(18), (4), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(21), (14), (8), (7), (6), (4), (3), (2), (2)
Pubmed 5 references available
Quantity 400 µL
Options
Shipping to United States (Change)
Availability Discontinued
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN656092
291.50 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 AA from the C-terminal region of human NKD2.
Clone RB31974
Isotype Ig
Specificity This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-401 amino acids from the C-terminal region of human NKD2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NKD2
Background In the mouse, Nkd is a Dishevelled (see DVL1, MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1, MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.
Synonyms: NKD2,Protein naked cuticle homolog 2,
Molecular Weight 50055 DA
Gene ID 85409
UniProt NP_149111.1, Q969F2
Research Area Ubiquitin-related antibodies, Cell Signaling, Cell Structure, Protein Modifications
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody NKD2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NKD2 antibody detected the NKD2 protein (arrow).
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody (2) NKD2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NKD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody (3) Confocal immunofluorescent analysis of NKD2 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term), (AA 366-393) antibody (4) NKD2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Background publications Li, Franklin, Graves-Deal et al.: "Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 15, pp. 5571-6, 2004 (PubMed).

Adamowicz, Radlwimmer, Rieker et al.: "Frequent amplifications and abundant expression of TRIO, NKD2, and IRX2 in soft tissue sarcomas." in: Genes, chromosomes & cancer, Vol. 45, Issue 9, pp. 829-38, 2006 (PubMed).

Li, Hao, Cao et al.: "Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells." in: Molecular biology of the cell, Vol. 18, Issue 8, pp. 3081-93, 2007 (PubMed).

Ding, Li, Hu et al.: "EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 36, pp. 13433-8, 2008 (PubMed).

Hu, Li, Cao et al.: "Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane." in: The Journal of biological chemistry, Vol. 285, Issue 18, pp. 13561-8, 2010 (PubMed).

Validation Images
back to top