Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) (C-Term), (AA 214-243) antibody

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Antigen
Synonyms FCNH, HAKA1
Epitope
C-Term, AA 214-243
(4), (2), (2), (1), (1)
Reactivity
Human
(14)
Host
Rabbit
(11), (4)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(14), (7), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN656492
Quantity 400 µL
Price
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Immunogen This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-243 AA from the C-terminal region of human FCN3.
Clone RB31101
Isotype Ig
Specificity This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-243 amino acids from the C-terminal region of human FCN3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name FCN3
Background Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified.
Synonyms: Ficolin-3,FCN3,FCNH, HAKA1
Molecular Weight 32903 DA
Gene ID 8547
UniProt O75636, NP_775628.1, NP_003656.2
Research Area Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.27 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment FCN3 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) (C-Term), (AA 214-243) antibody FCN3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FCN3 antibody detected the FCN3 protein (arrow).
anti-Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) (C-Term), (AA 214-243) antibody (2) FCN3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FCN3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) (C-Term), (AA 214-243) antibody (3) FCN3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Background publications Munthe-Fog, Hummelshøj, Ma et al.: "Characterization of a polymorphism in the coding sequence of FCN3 resulting in a Ficolin-3 (Hakata antigen) deficiency state." in: Molecular immunology, Vol. 45, Issue 9, pp. 2660-6, 2008 (PubMed).

Lacroix, Dumestre-Pérard, Schoehn et al.: "Residue Lys57 in the collagen-like region of human L-ficolin and its counterpart Lys47 in H-ficolin play a key role in the interaction with the mannan-binding lectin-associated serine proteases and the collectin receptor calreticulin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 456-65, 2008 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Hosts (11), (4)
Reactivities (14)
Applications (14), (7), (4), (2), (2), (2), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1)
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