UPF3 Regulator of Nonsense Transcripts Homolog A (Yeast) (UPF3A) (Center) antibody

Antigen: UPF3 Regulator of Nonsense Transcripts Homolog A (Yeast) (UPF3A)

Synonyms: UPF3, HUPF3A, RENT3A, MGC151365, 2600001C03Rik, 4930546M19Rik, UPF3A, si:dkey-21o13.6

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656510

Additional Information

Alternative name: UPF3A

Gene ID: 65110

UniProt: NP_075387.1, NP_542418.1, Q9H1J1

Immunogen: This UPF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-270 amino acids from the Central region of human UPF3A.

Isotype: Ig

Description: Other names: Regulator of nonsense transcripts 3A,UPF3A,RENT3A, UPF3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UPF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-270 amino acids from the Central region of human UPF3A.

Molecular Weight: 54696 DA

Comments:

Background: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: UPF3A Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Tarpey, Raymond, Nguyen et al.: "Mutations in UPF3B, a member of the nonsense-mediated mRNA decay complex, cause syndromic and nonsyndromic mental retardation." in: Nature genetics, Vol. 39, Issue 9, pp. 1127-33, 2007 (PubMed).