Variable Charge, X-Linked 2 (VCX2) (N-Term) antibody

Antigen: Variable Charge, X-Linked 2 (VCX2)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656641

Additional Information

Alternative name: VCX2

Gene ID: 51480

UniProt: Q9H322, NP_057462.2

Immunogen: This VCX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VCX2.

Isotype: Ig

Description: Other names: Variable charge X-linked protein 2,VCX2,VCX2R, VCXB

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VCX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VCX2.

Molecular Weight: 14661 DA

Comments:

Background: This gene belongs to the VCX/Y gene family, which has multiple members on both X and Y chromosomes, and all are expressed exclusively in male germ cells. The X-linked members are clustered on chromosome Xp22 and Y-linked members are two identical copies of the gene within a palindromic region on Yq11. The family members share a high degree of sequence identity, with the exception that a 30-bp unit is tandemly repeated in X-linked members but occurs only once in Y-linked members. The VCX gene cluster is polymorphic in terms of copy number, different individuals may have a different number of VCX genes. VCX/Y genes encode small and highly charged proteins of unknown function. The presence of a putative bipartite nuclear localization signal suggests that VCX/Y members are nuclear proteins. This gene contains 2 repeats of the 30-bp unit. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: VCX2 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Structure

Restrictions: For Research Use only

Images

VCX2 Antibody (N-term) (ABIN656641) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VCX2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Lahn, Page: "A human sex-chromosomal gene family expressed in male germ cells and encoding variably charged proteins." in: Human molecular genetics, Vol. 9, Issue 2, pp. 311-9, 2000 (PubMed).

Fukami, Kirsch, Schiller et al.: "A member of a gene family on Xp22.3, VCX-A, is deleted in patients with X-linked nonspecific mental retardation." in: American journal of human genetics, Vol. 67, Issue 3, pp. 563-73, 2000 (PubMed).

Suzuki, Tsunoda, Sese et al.: "Identification and characterization of the potential promoter regions of 1031 kinds of human genes." in: Genome research, Vol. 11, Issue 5, pp. 677-84, 2001 (PubMed).

Need, Attix, McEvoy et al.: "A genome-wide study of common SNPs and CNVs in cognitive performance in the CANTAB." in: Human molecular genetics, Vol. 18, Issue 23, pp. 4650-61, 2009 (PubMed).