WIPI2 antibody (N-Term)

Catalog No. ABIN657237

Product Details anti-WIPI2 Antibody

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Binding Specificity: AA 4-32, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WIPI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This WIPI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human WIPI2.

Clone: RB33312

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-WIPI2 antibody (ABIN657237)

Burbulla, Fitzgerald, Stegen, Westermeier, Thost, Kato, Mokranjac, Sauerwald, Martins, Woitalla, Rapaport, Riess, Proikas-Cezanne, Rasse, Krüger: "Mitochondrial proteolytic stress induced by loss of mortalin function is rescued by Parkin and PINK1." in: Cell death & disease, Vol. 5, pp. e1180, (2014) (PubMed).

Nemazanyy, Blaauw, Paolini, Caillaud, Protasi, Mueller, Proikas-Cezanne, Russell, Guan, Nishino, Sandri, Pende, Panasyuk: "Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease." in: EMBO molecular medicine, Vol. 5, Issue 6, pp. 870-90, (2013) (PubMed).

Target Details for WIPI2

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Alternative Name: WIPI2 (WIPI2 Products)

Synonyms: ATG18B antibody, Atg21 antibody, WIPI-2 antibody, 1110018O08Rik antibody, 2510001I10Rik antibody, WD repeat domain, phosphoinositide interacting 2 L homeolog antibody, WD repeat domain, phosphoinositide interacting 2 antibody, wipi2.L antibody, WIPI2 antibody, Wipi2 antibody

Background: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).

Molecular Weight: 49408

NCBI Accession: NP_001028690, NP_001028691, NP_001028692, NP_001265228, NP_056425, NP_057087

UniProt: Q9Y4P8