Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (C-Term) antibody

Antigen: Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A)

Synonyms: EIF2A, MGC165771, CDA02, EIF-2A, MST089, MSTP004, MSTP089, eif2alpha, Eif2a

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657642

Additional Information

Alternative name: EIF2A

Gene ID: 83939

UniProt: Q9BY44, NP_114414.2

Immunogen: This EIF2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-508 amino acids from the C-terminal region of human EIF2A.

Isotype: Ig

Description: Other names: Eukaryotic translation initiation factor 2A,EIF2A,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EIF2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-508 amino acids from the C-terminal region of human EIF2A.

Molecular Weight: 64990 DA

Comments:

Background: EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: EIF2A Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

EIF2A Antibody (C-term) (ABIN657642) western blot analysis in Ramos cell line lysates (35ug/lane). This demonstrates the EIF2A antibody detected the EIF2A protein (arrow).

Publications

Product: Spurgeon, Ornelles: "The adenovirus E1B 55-kilodalton and E4 open reading frame 6 proteins limit phosphorylation of eIF2alpha during the late phase of infection." in: Journal of virology, Vol. 83, Issue 19, pp. 9970-82, 2009 (PubMed).

Groskreutz, Babor, Monick et al.: "Respiratory syncytial virus limits alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) phosphorylation to maintain translation and viral replication." in: The Journal of biological chemistry, Vol. 285, Issue 31, pp. 24023-31, 2010 (PubMed).