Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) (Center) antibody

Antigen: Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H)

Synonyms: CDC68, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68, CG1828, DmelCG1828, DRE4/dSPT16, dspt16, dSpt16, dSPT16, l(3)62Bg, l(3)dre4, spt16, Spt16, Cdc68, Fact140, gtc102, global transcription factor C, SPT16, T12H20.3, T12H20_3, SSF1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657765

Additional Information

Alternative name: SUPT16H

Gene ID: 11198

UniProt: NP_009123.1, Q9Y5B9

Immunogen: This SUPT16H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-399 amino acids from the Central region of human SUPT16H.

Isotype: Ig

Description: Other names: FACT complex subunit SPT16,SUPT16H,FACT140, FACTP140

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SUPT16H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-399 amino acids from the Central region of human SUPT16H.

Molecular Weight: 119914 DA

Comments:

Background: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, this gene encodes the 140 kDa subunit. [provided by RefSeq].

Synonyms: CDC68, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68, CG1828, DmelCG1828, DRE4/dSPT16, dspt16, dSpt16, dSPT16, l(3)62Bg, l(3)dre4, spt16, Spt16, Cdc68, Fact140, gtc102, global transcription factor C, SPT16, T12H20.3, T12H20_3, SSF1

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: SUPT16H Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

SUPT16H Antibody (Center) (ABIN657765) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the SUPT16H antibody detected the SUPT16H protein (arrow).

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Heo, Kim, Choi et al.: "FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16." in: Molecular cell, Vol. 30, Issue 1, pp. 86-97, 2008 (PubMed).