RM33 (C-Term), (AA 36-65) antibody

Details for Product No. ABIN657799
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Antigen
Epitope
C-Term, AA 36-65
(1), (1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN657799
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Immunogen This RM33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 AA from the C-terminal region of human RM33.
Clone RB33988
Isotype Ig
Specificity This RM33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-65 amino acids from the C-terminal region of human RM33.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternatively spliced transcript variants encoding different isoforms have been described.
Synonyms: 39S ribosomal protein L33, mitochondrial,MRPL33,C2orf1
Molecular Weight 7619 DA
Gene ID 9553
NCBI Accession NP_004882
UniProt O75394
Research Area Translation Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment RM33 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-RM33 (C-Term), (AA 36-65) antibody RM33 Antibody (C-term) (ABIN657799) western blot analysis in HepG2 cell line lysates (35 µg/lane). This demonstrates the RM33 antibody detected the RM33 protein (arrow).
Background publications Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

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