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SFPQ antibody (Splicing Factor Proline/glutamine-Ric) (AA 676-705)

Details for Product anti-SFPQ Antibody No. ABIN657824, Supplier: Log in to see
Antigen
  • 1110004P21Rik
  • 2810416M14Rik
  • 5730453G22Rik
  • 9030402K04Rik
  • AU021830
  • D4Ertd314e
  • Gm12940
  • hm:zeh0027
  • OTTMUSG00000009329
  • POMP100
  • PSF
  • REP1
  • wu:fa11h12
  • wu:fd10f03
  • zgc:85935
Epitope
AA 676-705, C-Term
17
15
12
4
4
3
3
3
2
1
1
1
1
Reactivity
Human
73
17
11
3
2
1
1
1
Host
Rabbit
48
21
5
Clonality (Clone)
Polyclonal ()
Conjugate
This SFPQ antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
62
35
24
17
16
4
4
1
1
1
Supplier
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Immunogen This SFPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 676-705 AA from the C-terminal region of human SFPQ.
Clone RB34049
Isotype Ig
Specificity This SFPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 675-705 amino acids from the C-terminal region of human SFPQ.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SFPQ (SFPQ Antibody Abstract)
Background DNA-and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing, in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1, in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends, in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
Synonyms: Splicing factor, proline- and glutamine-rich,SFPQ,PSF
Molecular Weight 76149 DA
Gene ID 6421
NCBI Accession NP_005057
UniProt P23246
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment SFPQ Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-SFPQ antibody (Splicing Factor Proline/glutamine-Ric) (AA 676-705) (ABIN657824) SFPQ Antibody (C-term) (ABIN657824) western blot analysis in NCI-H460 cell line lysat...
Background publications Heyd, Lynch: "Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing." in: Molecular cell, Vol. 40, Issue 1, pp. 126-37, 2010 (PubMed).