Wingless-Type MMTV Integration Site Family, Member 8B (WNT8B) (C-Term) antibody

Antigen: Wingless-Type MMTV Integration Site Family, Member 8B (WNT8B)

Synonyms: wnt-8b, xwnt8b, xwnt-8b, WNT8B

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657869

Additional Information

Alternative name: WNT8B

Gene ID: 7479

UniProt: NP_003384.2, Q93098

Immunogen: This WNT8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-351 amino acids from the C-terminal region of human WNT8B.

Isotype: Ig

Description: Other names: Protein Wnt-8b,7479,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WNT8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-351 amino acids from the C-terminal region of human WNT8B.

Molecular Weight: 38721 DA

Comments:

Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: WNT8B Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

WNT8B Antibody (C-term) (ABIN657869) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the WNT8B antibody detected the WNT8B protein (arrow).

Publications

Product: Saitoh, Mine, Katoh: "Up-regulation of WNT8B mRNA in human gastric cancer." in: International journal of oncology, Vol. 20, Issue 2, pp. 343-8, 2002 (PubMed).

Saitoh, Mine, Katoh: "Expression and regulation of WNT8A and WNT8B mRNAs in human tumor cell lines: up-regulation of WNT8B mRNA by beta-estradiol in MCF-7 cells, and down-regulation of WNT8A and WNT8B mRNAs by retinoic acid in NT2 cells." in: International journal of oncology, Vol. 20, Issue 5, pp. 999-1003, 2002 (PubMed).

Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).

Grupe, Li, Rowland et al.: "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease." in: American journal of human genetics, Vol. 78, Issue 1, pp. 78-88, 2005 (PubMed).

Katoh, Katoh: "Conserved POU/OCT- and GATA-binding sites in 5'-flanking promoter region of mammalian WNT8B orthologs." in: International journal of oncology, Vol. 30, Issue 5, pp. 1273-7, 2007 (PubMed).