Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3) (C-Term) antibody

Antigen: Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3)

Synonyms: E2A, ITF1, VDIR, bHLHb21, MGC129647, MGC129648, tcf3a, MGC113045

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657912

Additional Information

Alternative name: TCF3

Gene ID: 6929

UniProt: NP_003191.1, NP_001129611.1, P15923

Immunogen: This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505-534 amino acids from the C-terminal region of human TCF3.

Isotype: Ig

Description: Other names: Transcription factor E2-alpha,6929,BHLHB21, E2A, ITF1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505-534 amino acids from the C-terminal region of human TCF3.

Molecular Weight: 67600 DA

Comments:

Background: The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: TCF3 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

TCF3 Antibody (C-term) (ABIN657912) western blot analysis in mouse cerebellum tissue lysates (35ug/lane). This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).

Publications

Product: Pedraza, Rafel, Navarro et al.: "Mixed lineage kinase phosphorylates transcription factor E47 and inhibits TrkB expression to link neuronal death and survival pathways." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32980-8, 2009 (PubMed).

Hauser, Wallenius, Sveshnikova et al.: "Calmodulin inhibition of E2A stops expression of surrogate light chains of the pre-B-cell receptor and CD19." in: Molecular immunology, Vol. 47, Issue 5, pp. 1031-8, 2010 (PubMed).

Rittié, Stoll, Kang et al.: "Hedgehog signaling maintains hair follicle stem cell phenotype in young and aged human skin." in: Aging cell, Vol. 8, Issue 6, pp. 738-51, 2009 (PubMed).

Pan, Yang, Chen et al.: "Impact of female cigarette smoking on circulating B cells in vivo: the suppressed ICOSLG, TCF3, and VCAM1 gene functional network may inhibit normal cell function." in: Immunogenetics, Vol. 62, Issue 4, pp. 237-51, 2010 (PubMed).

Hirose, Inukai, Kikuchi et al.: "Aberrant induction of LMO2 by the E2A-HLF chimeric transcription factor and its implication in leukemogenesis of B-precursor ALL with t(17;19)." in: Blood, Vol. 116, Issue 6, pp. 962-70, 2010 (PubMed).