Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (C-Term) antibody

Antigen: Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1)

Synonyms: PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C130083N04Rik, Prkaa1, MGC165837, cb116, sb:cb130, im:7154392, wu:fa94c10, DKFZp469M1511

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658004

Additional Information

Alternative name: Prkaa1

Gene ID: 105787

UniProt: Q5EG47, NP_001013385.3

Immunogen: This Mouse Prkaa1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-518 amino acids from the C-terminal region of human Mouse Prkaa1.

Isotype: Ig

Description: Other names: 5'-AMP-activated protein kinase catalytic subunit alpha-1,105787,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Mouse Prkaa1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-518 amino acids from the C-terminal region of human Mouse Prkaa1.

Molecular Weight: 63929 DA

Comments:

Background: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit (By similarity).

Synonyms: PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C130083N04Rik, Prkaa1, MGC165837, cb116, sb:cb130, im:7154392, wu:fa94c10, DKFZp469M1511

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Mouse Prkaa1 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Mouse Prkaa1 Antibody (C-term) (ABIN658004) western blot analysis in mouse kidney tissue lysates (35ug/lane). This demonstrates the Prkaa1 antibody detected the Prkaa1 protein (arrow).

Publications

Product: Randriamboavonjy, Isaak, Frömel et al.: "AMPK α2 subunit is involved in platelet signaling, clot retraction, and thrombus stability." in: Blood, Vol. 116, Issue 12, pp. 2134-40, 2010 (PubMed).

Foretz, Hébrard, Leclerc et al.: "Metformin inhibits hepatic gluconeogenesis in mice independently of the LKB1/AMPK pathway via a decrease in hepatic energy state." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2355-69, 2010 (PubMed).

Bungard, Fuerth, Zeng et al.: "Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation." in: Science (New York, N.Y.), Vol. 329, Issue 5996, pp. 1201-5, 2010 (PubMed).

Santidrián, González-Gironès, Iglesias-Serret et al.: "AICAR induces apoptosis independently of AMPK and p53 through up-regulation of the BH3-only proteins BIM and NOXA in chronic lymphocytic leukemia cells." in: Blood, Vol. 116, Issue 16, pp. 3023-32, 2010 (PubMed).

Foretz, Guihard, Leclerc et al.: "Maintenance of red blood cell integrity by AMP-activated protein kinase alpha1 catalytic subunit." in: FEBS letters, Vol. 584, Issue 16, pp. 3667-71, 2010 (PubMed).