Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (C-Term) antibody

Antigen: Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1)

Synonyms: PPK, KLK3, APS, PSA, Kal3, Klk3, Kal-3, Kalp1, KALP15, MGC108748, KLKB1, DKFZp459J2015

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658030

Additional Information

Alternative name: KLKB1

Gene ID: 3818

UniProt: P03952, NP_000883.2

Immunogen: This KLKB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 507-537 amino acids from the C-terminal region of human KLKB1.

Isotype: Ig

Description: Other names: Plasma kallikrein,KLKB1,KLK3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KLKB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 507-537 amino acids from the C-terminal region of human KLKB1.

Molecular Weight: 71370 DA

Comments:

Background: Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: KLKB1 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

KLKB1 Antibody (C-term) (ABIN658030) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the KLKB1 antibody detected the KLKB1 protein (arrow).

Publications

Product: Rajaraman, Brenner, Neta et al.: "Risk of meningioma and common variation in genes related to innate immunity." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 5, pp. 1356-61, 2010 (PubMed).