Cofilin 2 (Muscle) (CFL2) (N-Term) antibody

Antigen: Cofilin 2 (Muscle) (CFL2)

Synonyms: NEM7, CFL2, wu:fb17d06, wu:fb18d11, wu:fd59f08, wu:fj34b08, xac2, MGC53097, MGC77288, zgc:77288, cfl2, MGC140740, cf12

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658038

Additional Information

Alternative name: CFL2

Gene ID: 1073

UniProt: Q9Y281, NP_619579.1, NP_068733.1

Immunogen: This CFL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human CFL2.

Isotype: Ig

Description: Other names: Cofilin-2,CFL2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CFL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human CFL2.

Molecular Weight: 18737 DA

Comments:

Background: This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: CFL2 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Publications

Product: Agrawal, Greenleaf, Tomczak et al.: "Nemaline myopathy with minicores caused by mutation of the CFL2 gene encoding the skeletal muscle actin-binding protein, cofilin-2." in: American journal of human genetics, Vol. 80, Issue 1, pp. 162-7, 2006 (PubMed).

Vogel, Wottawa, Farhat et al.: "Prolyl hydroxylase domain (PHD) 2 affects cell migration and F-actin formation via RhoA/rho-associated kinase-dependent cofilin phosphorylation." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 33756-63, 2010 (PubMed).