Mitogen-Activated Protein Kinase 6 (MAPK6) (Center) antibody

Antigen: Mitogen-Activated Protein Kinase 6 (MAPK6)

Synonyms: ERK3, PRKM6, p97MAPK, HsT17250, DKFZp686F03189, MAPK6, DKFZp459L0825

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658057

Additional Information

Alternative name: Mapk6

Gene ID: 50772

UniProt: NP_081694.1, Q61532, NP_056621.4

Immunogen: This Mouse Mapk6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388-418 amino acids from the Central region of human Mouse Mapk6.

Isotype: Ig

Description: Other names: Mitogen-activated protein kinase 6,Mapk6,Erk3, Prkm4, Prkm6

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Mouse Mapk6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388-418 amino acids from the Central region of human Mouse Mapk6.

Molecular Weight: 82199 DA

Comments:

Background: Mapk6 phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Mouse Mapk6 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Mouse Mapk6 Antibody (Center) (ABIN658057) western blot analysis in mouse cerebellum tissue lysates (35ug/lane). This demonstrates the Mapk6 antibody detected the Mapk6 protein (arrow).

Publications

Product: Schumacher, Laass, Kant et al.: "Scaffolding by ERK3 regulates MK5 in development." in: The EMBO journal, Vol. 23, Issue 24, pp. 4770-9, 2004 (PubMed).

Seternes, Mikalsen, Johansen et al.: "Activation of MK5/PRAK by the atypical MAP kinase ERK3 defines a novel signal transduction pathway." in: The EMBO journal, Vol. 23, Issue 24, pp. 4780-91, 2004 (PubMed).

Klinger, Turgeon, Lévesque et al.: "Loss of Erk3 function in mice leads to intrauterine growth restriction, pulmonary immaturity, and neonatal lethality." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 39, pp. 16710-5, 2009 (PubMed).