Integrin alpha-8 / ITGA8 (Center) antibody

Antigen: Integrin alpha-8 / ITGA8

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658098

Additional Information

Alternative name: ITGA8

Gene ID: 8516

UniProt: NP_003629.1, P53708

Immunogen: This ITGA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 570-599 amino acids from the Central region of human ITGA8.

Isotype: Ig

Description: Other names: Integrin alpha-8,ITGA8,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ITGA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 570-599 amino acids from the Central region of human ITGA8.

Molecular Weight: 117474 DA

Comments:

Background: Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: ITGA8 Antibody (Center) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Neurology

Restrictions: For Research Use only

Images

ITGA8 Antibody (Center) (ABIN658098) western blot analysis in A549 cell line lysates (35ug/lane). This demonstrates the ITGA8 antibody detected the ITGA8 protein (arrow).

Publications

Product: Sato, Uemura, Morimitsu et al.: "Molecular basis of the recognition of nephronectin by integrin alpha8beta1." in: The Journal of biological chemistry, Vol. 284, Issue 21, pp. 14524-36, 2009 (PubMed).

Benoit, Lussier, Ducharme et al.: "Integrin alpha8beta1 regulates adhesion, migration and proliferation of human intestinal crypt cells via a predominant RhoA/ROCK-dependent mechanism." in: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 101, Issue 12, pp. 695-708, 2009 (PubMed).

Simón-Sánchez, Schulte, Bras et al.: "Genome-wide association study reveals genetic risk underlying Parkinson's disease." in: Nature genetics, Vol. 41, Issue 12, pp. 1308-12, 2009 (PubMed).

Benoit, Larrivée, Groulx et al.: "Integrin alpha8beta1 confers anoikis susceptibility to human intestinal epithelial crypt cells." in: Biochemical and biophysical research communications, Vol. 399, Issue 3, pp. 434-9, 2010 (PubMed).