Enhancer of Zeste Homolog 2 (Drosophila) (EZH1) (N-Term) antibody

Antigen: Enhancer of Zeste Homolog 2 (Drosophila) (EZH1)

Synonyms: eya2, MGC136376, im:7149338, zgc:136376, zgc:152758, MGC189404, EZH2, ezh2, KMT6, Enx-1, Enx1h, KIAA4065, MGC90723, mKIAA4065, KIAA0388, ENX1, EZH1, ENX-1, MGC9169, Ezh2, xez, ezh2-A, MGC79865, MGC136829, zgc:136829, olezh2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658118

Additional Information

Alternative name: EZH2

Gene ID: 2146

UniProt: NP_004447.2, NP_694543.1, Q15910

Immunogen: This EZH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-212 amino acids from the N-terminal region of human EZH2.

Isotype: Ig

Description: Other names: Histone-lysine N-methyltransferase EZH2,EZH2,KMT6

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EZH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-212 amino acids from the N-terminal region of human EZH2.

Molecular Weight: 85363 DA

Comments:

Background: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.

Synonyms: eya2, MGC136376, im:7149338, zgc:136376, zgc:152758, MGC189404, EZH2, ezh2, KMT6, Enx-1, Enx1h, KIAA4065, MGC90723, mKIAA4065, KIAA0388, ENX1, EZH1, ENX-1, MGC9169, Ezh2, xez, ezh2-A, MGC79865, MGC136829, zgc:136829, olezh2

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: EZH2 Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

EZH2 Antibody (N-term) (ABIN658118) western blot analysis in MDA-MB468 cell line lysates (35ug/lane). This demonstrates the EZH2 antibody detected the EZH2 protein (arrow).

Publications

Product: Ernst, Chase, Score et al.: "Inactivating mutations of the histone methyltransferase gene EZH2 in myeloid disorders." in: Nature genetics, Vol. 42, Issue 8, pp. 722-6, 2010 (PubMed).

Nikoloski, Langemeijer, Kuiper et al.: "Somatic mutations of the histone methyltransferase gene EZH2 in myelodysplastic syndromes." in: Nature genetics, Vol. 42, Issue 8, pp. 665-7, 2010 (PubMed).

Tsai, Manor, Wan et al.: "Long noncoding RNA as modular scaffold of histone modification complexes." in: Science (New York, N.Y.), Vol. 329, Issue 5992, pp. 689-93, 2010 (PubMed).