High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN658140
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Antigen
Synonyms hmg14, hmgn1, hmgn1b, HMG-14, Hmg14, HMG14
Epitope
N-Term, AA 1-30
(17), (17), (8), (7), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(61), (40), (39), (28), (27), (25), (25), (2), (1), (1)
Host
Rabbit
(63)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(28), (27), (20), (19), (18), (16), (10), (6), (2), (2)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN658140
291.50 $
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Immunogen This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human HMGN1.
Clone RB34198
Isotype Ig
Specificity This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HMGN1
Background Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.
Synonyms: Non-histone chromosomal protein HMG-14,HMGN1,HMG14
Molecular Weight 10659 DA
Gene ID 3150
NCBI Accession NP_004956
UniProt P05114
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment HMGN1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (N-Term), (AA 1-30) antibody HMGN1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGN1 antibody detected the HMGN1 protein (arrow).
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (N-Term), (AA 1-30) antibody (2) Western blot analysis of HMGN1 (arrow) using rabbit polyclonal HMGN1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN1 gene.
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (N-Term), (AA 1-30) antibody (3) HMGN1 Antibody (N-term) (AP14219a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Background publications Jiang, Wang: "Phosphorylation of human high mobility group N1 protein by protein kinase CK2." in: Biochemical and biophysical research communications, Vol. 345, Issue 4, pp. 1497-503, 2006 (PubMed).

Zhu, Hansen: "HMGN1 modulates estrogen-mediated transcriptional activation through interactions with specific DNA-binding transcription factors." in: Molecular and cellular biology, Vol. 27, Issue 24, pp. 8859-73, 2007 (PubMed).

Cherukuri, Hock, Ueda et al.: "Cell cycle-dependent binding of HMGN proteins to chromatin." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 1816-24, 2008 (PubMed).

Rattner, Yusufzai, Kadonaga: "HMGN proteins act in opposition to ATP-dependent chromatin remodeling factors to restrict nucleosome mobility." in: Molecular cell, Vol. 34, Issue 5, pp. 620-6, 2009 (PubMed).

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