High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (N-Term) antibody

Antigen: High Mobility Group Nucleosome Binding Domain 1 (HMGN1)

Synonyms: hmg14, hmgn1b, MGC64449, MGC75785, HMG14, FLJ27265, FLJ31471, MGC104230, MGC117425, Hmg14, HMG-14, MGC103397, MGC127465

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658140

Additional Information

Alternative name: HMGN1

Gene ID: 3150

UniProt: P05114, NP_004956.5

Immunogen: This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.

Isotype: Ig

Description: Other names: Non-histone chromosomal protein HMG-14,HMGN1,HMG14

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.

Molecular Weight: 10659 DA

Comments:

Background: Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: HMGN1 Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Publications

Product: Jiang, Wang: "Phosphorylation of human high mobility group N1 protein by protein kinase CK2." in: Biochemical and biophysical research communications, Vol. 345, Issue 4, pp. 1497-503, 2006 (PubMed).

Zhu, Hansen: "HMGN1 modulates estrogen-mediated transcriptional activation through interactions with specific DNA-binding transcription factors." in: Molecular and cellular biology, Vol. 27, Issue 24, pp. 8859-73, 2007 (PubMed).

Cherukuri, Hock, Ueda et al.: "Cell cycle-dependent binding of HMGN proteins to chromatin." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 1816-24, 2008 (PubMed).

Rattner, Yusufzai, Kadonaga: "HMGN proteins act in opposition to ATP-dependent chromatin remodeling factors to restrict nucleosome mobility." in: Molecular cell, Vol. 34, Issue 5, pp. 620-6, 2009 (PubMed).