|+1 404 474 4654|
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High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (N-Term) antibody
|Synonyms||hmg14, hmgn1b, MGC64449, MGC75785, HMG14, FLJ27265, FLJ31471, MGC104230, MGC117425, Hmg14, HMG-14, MGC103397, MGC127465|
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.|
|Description||Other names: Non-histone chromosomal protein HMG-14,HMGN1,HMG14|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.|
|Molecular Weight||10659 DA|
Background: Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||HMGN1 Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.|
|Research Area||Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
Jiang, Wang: "Phosphorylation of human high mobility group N1 protein by protein kinase CK2." in: Biochemical and biophysical research communications, Vol. 345, Issue 4, pp. 1497-503, 2006 (PubMed).
Zhu, Hansen: "HMGN1 modulates estrogen-mediated transcriptional activation through interactions with specific DNA-binding transcription factors." in: Molecular and cellular biology, Vol. 27, Issue 24, pp. 8859-73, 2007 (PubMed).
Cherukuri, Hock, Ueda et al.: "Cell cycle-dependent binding of HMGN proteins to chromatin." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 1816-24, 2008 (PubMed).
Rattner, Yusufzai, Kadonaga: "HMGN proteins act in opposition to ATP-dependent chromatin remodeling factors to restrict nucleosome mobility." in: Molecular cell, Vol. 34, Issue 5, pp. 620-6, 2009 (PubMed).
|Reactivities||Human (26), Mouse (Murine) (19), Rat (Rattus) (18), Cow (Bovine) (14), Dog (Canine) (14), Chicken (12), Pig (Porcine) (11), Rabbit (2), Cat (Feline) (1), Frog (1), Monkey (1)|
|Applications||Immunofluorescence (IF) (16), ELISA (13), Western Blotting (WB) (13), Immunohistochemistry (IHC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunocytochemistry (ICC) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2)|
|Epitopes||N-Term (6), pSer24,pSer20 (6), Internal Region (2), N-Term,AA 1-30 (1), pSer20,pSer24 (1), pSer21 (1)|