Uromodulin (UMOD) (Center) antibody

Antigen: Uromodulin (UMOD)

Synonyms: THP, FJHN, HNFJ, THGP, MCKD2, ADMCKD2, UMOD, DKFZp469L1231

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658177

Additional Information

Alternative name: UMOD

Gene ID: 7369

UniProt: NP_003352.2, P07911, NP_001008390.1

Immunogen: This UMOD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the Central region of human UMOD.

Isotype: Ig

Description: Other names: Uromodulin,UMOD,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UMOD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the Central region of human UMOD.

Molecular Weight: 69761 DA

Comments:

Background: This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: UMOD Antibody (Center) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

UMOD Antibody (Center) (ABIN658177) western blot analysis in MDA-MB453 cell line lysates (35ug/lane). This demonstrates the UMOD antibody detected the UMOD protein (arrow).

Publications

Product: Ashby, Kehoe, Love: "Kallikrein-related peptidase 6 in Alzheimer's disease and vascular dementia." in: Journal of the American Chemical Society, Vol. 1363, Issue Nucleic acids research, pp. 1-10, 2010

Pattaro, De Grandi, Vitart et al.: "A meta-analysis of genome-wide data from five European isolates reveals an association of COL22A1, SYT1, and GABRR2 with serum creatinine level." in: BMC medical genetics, Vol. 11, pp. 41, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).