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AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 227-256), (C-Term) antibody

Details for Product No. ABIN658196, Supplier: Login to see
Antigen
  • MGC81452
  • lobe
  • pras40
  • fb34a04
  • wu:fb34a04
  • Lobe
  • PRAS40
  • 1110012J22Rik
  • AI227026
  • Lobel
Alternatives
anti-Human AKT1 Substrate 1 (Proline-Rich) antibody for Western Blotting
Epitope
AA 227-256, C-Term
30
15
13
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
89
22
20
5
1
Host
Rabbit
68
21
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
3
3
3
3
3
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
81
38
27
9
8
4
3
Supplier
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Immunogen This AKT1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-256 AA from the C-terminal region of human AKT1S1.
Clone RB34859
Isotype Ig
Specificity This AKT1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human AKT1S1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name AKT1S1 (AKT1S1 Antibody Abstract)
Background AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].
Synonyms: Proline-rich AKT1 substrate 1,AKT1S1,PRAS40
Molecular Weight 27383 DA
Gene ID 84335
NCBI Accession NP_001092102, NP_001092103, NP_115751
Research Area Protein Modifications, Cell Structure, Phospho-specific antibodies
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment AKT1S1 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 227-256), (C-Term) antibody (ABIN658196) AKT1S1 Antibody (C-term) (ABIN658196) western blot analysis in Hela cell line lysates...
Background publications Wang, Harris, Lawrence: "Regulation of proline-rich Akt substrate of 40 kDa (PRAS40) function by mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15619-27, 2008 (PubMed).

Wang, Harris, Roth et al.: "PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding." in: The Journal of biological chemistry, Vol. 282, Issue 27, pp. 20036-44, 2007 (PubMed).

Sancak, Thoreen, Peterson et al.: "PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase." in: Molecular cell, Vol. 25, Issue 6, pp. 903-15, 2007 (PubMed).