AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (C-Term) antibody

Antigen: AKT1 Substrate 1 (Proline-Rich) (AKT1S1)

Synonyms: Lobe, Lobel, PRAS40, AI227026, 1110012J22Rik, MGC2865, MGC81452, AKT1S1, lobe, pras40, MGC148526, fb34a04, wu:fb34a04

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658196

Additional Information

Alternative name: AKT1S1

Gene ID: 84335

UniProt: NP_115751.2, NP_001092103.1, NP_001092102.1

Immunogen: This AKT1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human AKT1S1.

Isotype: Ig

Description: Other names: Proline-rich AKT1 substrate 1,AKT1S1,PRAS40

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AKT1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human AKT1S1.

Molecular Weight: 27383 DA

Comments:

Background: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: AKT1S1 Antibody (C-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Protein Modifications, Cell Structure, Phospho-specific antibodies

Restrictions: For Research Use only

Images

AKT1S1 Antibody (C-term) (ABIN658196) western blot analysis in Hela cell line lysates (35ug/lane). This demonstrates the AKT1S1 antibody detected the AKT1S1 protein (arrow).

Publications

Product: Sancak, Thoreen, Peterson et al.: "PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase." in: Molecular cell, Vol. 25, Issue 6, pp. 903-15, 2007 (PubMed).

Wang, Harris, Roth et al.: "PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding." in: The Journal of biological chemistry, Vol. 282, Issue 27, pp. 20036-44, 2007 (PubMed).

Wang, Harris, Lawrence: "Regulation of proline-rich Akt substrate of 40 kDa (PRAS40) function by mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15619-27, 2008 (PubMed).