TEA Domain Family Member 2 (TEAD2) (Center) antibody

Antigen: TEA Domain Family Member 2 (TEAD2)

Synonyms: ETF, TEF4, TEF-4, Etdf, TEAD-2, TEAD2, MGC139952

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658203

Additional Information

Alternative name: TEAD2

Gene ID: 8463

UniProt: NP_003589.1, Q15562

Immunogen: This TEAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human TEAD2.

Isotype: Ig

Description: Other names: Transcriptional enhancer factor TEF-4,TEAD2,TEF4

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TEAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human TEAD2.

Molecular Weight: 49243 DA

Comments:

Background: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: TEAD2 Antibody (Center) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Transcription Factors, Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

TEAD2 Antibody (Center) (ABIN658203) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane). This demonstrates the TEAD2 antibody detected the TEAD2 protein (arrow).

Publications

Product: Zhao, Ye, Yu et al.: "TEAD mediates YAP-dependent gene induction and growth control." in: Genes & development, Vol. 22, Issue 14, pp. 1962-71, 2008 (PubMed).

Zhang, Liu, Zha et al.: "TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13355-62, 2009 (PubMed).