Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) (Center) antibody

Antigen: Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2)

Synonyms: ELOB, SIII

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658243

Additional Information

Alternative name: TCEB2

Gene ID: 6923

UniProt: NP_009039.1, Q15370

Immunogen: This TCEB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the Central region of human TCEB2.

Isotype: Ig

Description: Other names: Transcription elongation factor B polypeptide 2,TCEB2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TCEB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the Central region of human TCEB2.

Molecular Weight: 13133 DA

Comments:

Background: This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: TCEB2 Antibody (Center) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Signaling, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

TCEB2 Antibody (Center) (ABIN658243) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the TCEB2 antibody detected the TCEB2 protein (arrow).

Publications

Product: Bullock, Debreczeni, Edwards et al.: "Crystal structure of the SOCS2-elongin C-elongin B complex defines a prototypical SOCS box ubiquitin ligase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 20, pp. 7637-42, 2006 (PubMed).

Van Herreweghe, Egloff, Goiffon et al.: "Dynamic remodelling of human 7SK snRNP controls the nuclear level of active P-TEFb." in: The EMBO journal, Vol. 26, Issue 15, pp. 3570-80, 2007 (PubMed).