APG8b (MAP1LC3B) (N-Term,Thr29) antibody

Antigen: APG8b (MAP1LC3B)

Binding Site: N-Term,Thr29

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658250

Additional Information

Gene ID: 81631

UniProt: NP_073729.1, Q9GZQ8

Immunogen: This APG8b(MAP1LC3B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-33 amino acids from the N-terminal region of human APG8b(MAP1LC3B).

Isotype: Ig

Description: Other names: Microtubule-associated proteins 1A/1B light chain 3B,MAP1LC3B,MAP1ALC3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This APG8b(MAP1LC3B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-33 amino acids from the N-terminal region of human APG8b(MAP1LC3B).

Molecular Weight: 14688 DA

Comments:

Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: APG8b(MAP1LC3B)Antibody (N-term T29) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Cell Structure, Autophagy

Restrictions: For Research Use only

Images

APG8b(MAP1LC3B)Antibody (N-term T29) (ABIN658250) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).

Publications

Product: Kirkin, Lamark, Sou et al.: "A role for NBR1 in autophagosomal degradation of ubiquitinated substrates." in: Molecular cell, Vol. 33, Issue 4, pp. 505-16, 2009 (PubMed).