Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibody

Antigen: Nuclear Transcription Factor Y, beta (NFYB)

Synonyms: HAP3, CBF-A, CBF-B, NF-YB, NFYB, zgc:110533, zgc:110552, hap3, cbf-a, cbf-b

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658255

Additional Information

Alternative name: NFYB

Gene ID: 4801

UniProt: P25208, NP_006157.1

Immunogen: This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human NFYB.

Isotype: Ig

Description: Other names: Nuclear transcription factor Y subunit beta,NFYB,HAP3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human NFYB.

Molecular Weight: 22831 DA

Comments:

Background: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: NFYB Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

NFYB Antibody (N-term) (ABIN658255) western blot analysis in A549 cell line lysates (35ug/lane). This demonstrates the NFYB antibody detected the NFYB protein (arrow).

Publications

Product: Di Agostino, Strano, Emiliozzi et al.: "Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation." in: Cancer cell, Vol. 10, Issue 3, pp. 191-202, 2006 (PubMed).

Raju, Göritz, Nord et al.: "In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 3008-19, 2008 (PubMed).

Stros, Polanská, Struncová et al.: "HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase IIalpha." in: Nucleic acids research, Vol. 37, Issue 7, pp. 2070-86, 2009 (PubMed).