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NFYB antibody (Nuclear Transcription Factor Y, beta) (AA 17-46)

Details for Product anti-NFYB Antibody No. ABIN658255, Supplier: Log in to see
Antigen
  • NFYB
  • hap3
  • NF-YB
  • cbf-a
  • cbf-b
  • CBF-A
  • CBF-B
  • HAP3
  • nfyb
  • zgc:110533
  • AA985999
  • Cbf-A
  • CBFB
  • Nfya
Epitope
AA 17-46, N-Term
36
15
6
6
3
1
1
1
1
1
Reactivity
Human
67
11
9
4
3
3
2
1
Host
Rabbit
49
18
Clonality (Clone)
Polyclonal ()
Conjugate
This NFYB antibody is un-conjugated
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
60
44
13
12
8
4
4
3
3
2
2
2
1
Supplier
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Immunogen This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 AA from the N-terminal region of human NFYB.
Clone RB30270
Isotype Ig
Specificity This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human NFYB.
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NFYB (NFYB Antibody Abstract)
Background The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.
Synonyms: Nuclear transcription factor Y subunit beta,NFYB,HAP3
Molecular Weight 22831 DA
Gene ID 4801
NCBI Accession NP_006157
UniProt P25208
Research Area Translation Factors, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.42 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment NFYB Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-NFYB antibody (Nuclear Transcription Factor Y, beta) (AA 17-46) (ABIN658255) NFYB Antibody (N-term) (ABIN658255) western blot analysis in A549 cell line lysates (...
Background publications Stros, Polanská, Struncová et al.: "HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase IIalpha." in: Nucleic acids research, Vol. 37, Issue 7, pp. 2070-86, 2009 (PubMed).

Raju, Göritz, Nord et al.: "In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 3008-19, 2008 (PubMed).

Di Agostino, Strano, Emiliozzi et al.: "Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation." in: Cancer cell, Vol. 10, Issue 3, pp. 191-202, 2006 (PubMed).