Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1) (Center) antibody

Antigen: Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658266

Additional Information

Alternative name: MARCH1

Gene ID: 55016

UniProt: NP_060393.1, NP_001159845.1, Q8TCQ1

Immunogen: This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human MARCH1.

Isotype: Ig

Description: Other names: E3 ubiquitin-protein ligase MARCH1,MARCH1,RNF171

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human MARCH1.

Molecular Weight: 32308 DA

Comments:

Background: MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266], Thibodeau et al., 2008 [PubMed 18389477], De Gassart et al., 2008 [PubMed 18305173]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: MARCH1 Antibody (Center) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Cell Signaling, Cell Structure, Chromatin, Signaling

Restrictions: For Research Use only

Images

MARCH1 Antibody (Center) (ABIN658266) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).

Publications

Product: Bartee, Mansouri, Hovey Nerenberg et al.: "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins." in: Journal of virology, Vol. 78, Issue 3, pp. 1109-20, 2004 (PubMed).

De Gassart, Camosseto, Thibodeau et al.: "MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3491-6, 2008 (PubMed).