Ubiquitin Specific Peptidase 15 (USP15) (N-Term) antibody

Antigen: Ubiquitin Specific Peptidase 15 (USP15)

Synonyms: USP15, UNPH4, KIAA0529, MGC74854, MGC131982, MGC149838, Gcap18, AI327321, mKIAA0529, 4921514G19Rik, E430033I05Rik, UBP43, Ubp15, AW047653, 1110058H21Rik, Ubp109, UNP, MGC128893

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658273

Additional Information

Alternative name: USP15

Gene ID: 9958

UniProt: NP_006304.1, Q9Y4E8

Immunogen: This USP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human USP15.

Isotype: Ig

Description: Other names: Ubiquitin carboxyl-terminal hydrolase 15,USP15,KIAA0529

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This USP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human USP15.

Molecular Weight: 112419 DA

Comments:

Background: Ubiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: USP15 Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

USP15 Antibody (N-term) (ABIN658273) western blot analysis in mouse cerebellum tissue lysates (35ug/lane). This demonstrates the USP15 antibody detected the USP15 protein (arrow).

Publications

Product: Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Vos, Altreuter, White et al.: "The ubiquitin-specific peptidase USP15 regulates human papillomavirus type 16 E6 protein stability." in: Journal of virology, Vol. 83, Issue 17, pp. 8885-92, 2009 (PubMed).

Huang, Langelotz, Hetfeld-Pechoc et al.: "The COP9 signalosome mediates beta-catenin degradation by deneddylation and blocks adenomatous polyposis coli destruction via USP15." in: Journal of molecular biology, Vol. 391, Issue 4, pp. 691-702, 2009 (PubMed).