protein ariadne-2 homolog (ARIH2) (C-Term) antibody

Antigen: protein ariadne-2 homolog (ARIH2)

Synonyms: ARI2, TRIAD1, FLJ10938, FLJ33921

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658296

Additional Information

Alternative name: ARIH2

Gene ID: 10425

UniProt: NP_006312.1, O95376

Immunogen: This ARIH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the C-terminal region of human ARIH2.

Isotype: Ig

Description: Other names: E3 ubiquitin-protein ligase ARIH2,ARIH2,ARI2, TRIAD1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ARIH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the C-terminal region of human ARIH2.

Molecular Weight: 57819 DA

Comments:

Background: E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: ARIH2 Antibody (C-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ARIH2 (arrow) using rabbit polyclonal ARIH2 Antibody (C-term) (ABIN658296). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARIH2 gene.

Publications

Product: Marteijn, van Emst, Erpelinck-Verschueren et al.: "The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth of primary myeloid progenitor cells." in: Blood, Vol. 106, Issue 13, pp. 4114-23, 2005 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Marteijn, van der Meer, van Emst et al.: "Gfi1 ubiquitination and proteasomal degradation is inhibited by the ubiquitin ligase Triad1." in: Blood, Vol. 110, Issue 9, pp. 3128-35, 2007 (PubMed).

Marteijn, van der Meer, Smit et al.: "The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 23, Issue 8, pp. 1480-9, 2009 (PubMed).