Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 2 (DYRK2) (C-Term) antibody

Antigen: Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 2 (DYRK2)

Synonyms: FLJ21217, FLJ21365, 1810038L18Rik, DYRK2, fc18c09, wu:fa09g03, wu:fc18c09, si:ch211-266a5.8

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658331

Additional Information

Alternative name: DYRK2

Gene ID: 8445

UniProt: Q92630, NP_006473.2, NP_003574.1

Immunogen: This DYRK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 466-495 amino acids from the C-terminal region of human DYRK2.

Isotype: Ig

Description: Other names: Dual specificity tyrosine-phosphorylation-regulated kinase 2,DYRK2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DYRK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 466-495 amino acids from the C-terminal region of human DYRK2.

Molecular Weight: 66652 DA

Comments:

Background: DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: DYRK2 Antibody (C-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

DYRK2 Antibody (C-term) (ABIN658331) western blot analysis in HL-60 cell line lysates (35ug/lane). This demonstrates the DYRK2 antibody detected the DYRK2 protein (arrow).

Publications

Product: Taira, Nihira, Yamaguchi et al.: "DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage." in: Molecular cell, Vol. 25, Issue 5, pp. 725-38, 2007 (PubMed).

Maddika, Chen: "Protein kinase DYRK2 is a scaffold that facilitates assembly of an E3 ligase." in: Nature cell biology, Vol. 11, Issue 4, pp. 409-19, 2009 (PubMed).

Taira, Yamamoto, Yamaguchi et al.: "ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4909-19, 2010 (PubMed).