Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (N-Term) antibody

Antigen: Karyopherin alpha 1 (Importin alpha 5) (KPNA1)

Synonyms: RCH2, SRP1, IPOA5, NPI-1, QIP2, RCH1, IPOA1, SRP1alpha, KPNA1, DKFZp459M124

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658337

Additional Information

Alternative name: KPNA1

Gene ID: 3836

UniProt: NP_002255.3, P52294

Immunogen: This KPNA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KPNA1.

Isotype: Ig

Description: Other names: Importin subunit alpha-1,KPNA1,RCH2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KPNA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KPNA1.

Molecular Weight: 60222 DA

Comments:

Background: Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: KPNA1 Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

KPNA1 Antibody (N-term) (ABIN658337) western blot analysis in Hela cell line lysates (35ug/lane). This demonstrates the KPNA1 antibody detected the KPNA1 protein (arrow).

Publications

Product: Simkus, Makiya, Jones: "Karyopherin alpha 1 is a putative substrate of the RAG1 ubiquitin ligase." in: Molecular immunology, Vol. 46, Issue 7, pp. 1319-25, 2009 (PubMed).

Yang, Takeda, Fontes et al.: "Probing the specificity of binding to the major nuclear localization sequence-binding site of importin-alpha using oriented peptide library screening." in: The Journal of biological chemistry, Vol. 285, Issue 26, pp. 19935-46, 2010 (PubMed).

OSeaghdha, Yang, Glazer et al.: "Common variants in the calcium-sensing receptor gene are associated with total serum calcium levels." in: Human molecular genetics, Vol. 19, Issue 21, pp. 4296-303, 2010 (PubMed).